![]() It has been suggested that HTLV-1–infected CD4 + T cells and HTLV-1–specific CD8 + cytotoxic T cells enter the CNS and release various proinflammatory cytokines, resulting in the neural damage and degeneration in HAM/TSP patients ( 7). The frequency of these Tax-specific CTLs is extraordinarily high in peripheral blood and even higher in cerebrospinal fluid (CSF) ( 6), in which virus-specific CTLs demonstrate degranulation activity and produce proinflammatory cytokines ( 3, 4). The HTLV-1 regulatory protein Tax promotes the proliferation of HTLV-1–infected lymphocytes and is an immunodominant antigen recognized by HTLV-1–specific CTLs ( 5). HTLV-1 predominantly infects CD4 + T cells and causes the activation and proliferation of infected cells, which express viral proteins that may lead to the activation and expansion of HTLV-1–specific CD8 + cytotoxic T lymphocytes (CTLs) ( 3, 4). ![]() HAM/TSP is a neuroinflammatory disease of the spinal cord with progressive spastic paraparesis and bladder dysfunction. Human T-lymphotropic type 1 (HTLV-1) infects 10 million–20 million people worldwide and is the etiologic agent of adult T cell leukemia/lymphoma ( 1) and an inflammatory neurologic disease, HTLV-1–associated myelopathy/tropical spastic paraparesis (HAM/TSP) ( 2). These results suggest that exploring TCR repertoires of CSF and antigen-specific T cells may provide a TCR repertoire signature in virus-associated neurologic disorders. Importantly, TCR β clonotypes of expanded clones in HTLV-1 Tax11-19–specific CD8 + T cells were also expanded and enriched in the CSF of the same patient. In addition, we analyzed TCR β repertoires of highly expanded and potentially immunopathologic HTLV-1 Tax11-19–specific CD8 + T cells from PBMCs of HLA-A*0201 + HAM/TSP and identified a conserved motif (PGLAG) in the CDR3 region. The sequence analysis demonstrated that TCR β repertoires in CSF of HAM/TSP patients were highly expanded and contained both TCR clonotypes shared with PBMCs and uniquely enriched within the CSF. TCR β libraries using unique molecular identifier–based methodologies were sequenced in paired peripheral blood mononuclear cells (PBMCs) and CSF cells from HAM/TSP patients and normal healthy donors (NDs). NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).In this study, we examined and characterized disease-specific TCR signatures in cerebrospinal fluid (CSF) of patients with HTLV-1–associated myelopathy/tropical spastic paraparesis (HAM/TSP). Our mission is to develop high-quality innovative tools and services to accelerate discovery.įOR RESEARCH USE ONLY. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. Illumina-ready: sequencing libraries can be multiplexed with up to 96 primer index options.Specific: optimized RACE-based PCR efficiently captures target region.Reproducible: consistent clonotype identification and isotype calling across a range of sample inputs.Sensitive: detect rare functional variants.Learn more about the kits and see sample data by viewing our tech notes. By avoiding multiplexed PCR reactions, these kits also give reproducible, sensitive, and unbiased results from a wide range of input amounts. Unlike methods that use DNA as a template, our kits identify expressed receptor sequences and provide greater sensitivity for a more comprehensive identification of unique functional variants. Our kits streamline sample preparation by combining our SMART ( Switching Mechanism at 5' End of RNA Template) technology with 5' RACE to capture V(D)J sequences, starting from RNA samples. We offer a variety of kits for T- and B-cell profiling of human ( bulk BCR, bulk TCR, single-cell TCR) and mouse ( bulk BCR, bulk TCR) samples to help researchers investigate immune cell development, examine mechanisms of autoimmune diseases and cancers, and develop models for immunotherapy of specific diseases. Immune profiling involves the analysis of T- and B-cell receptors (TCRs/BCRs), which allow cells to bind specific antigens, proliferate, and then rapidly mount a specific immune response.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |